STAGE I: CLEANING
Washing and cleaning of instruments are done by hand scrub method using detergent solution.
STAGE II: COLD STERILIZATION
All the instruments are cold sterilized by soaking them for one hour in a disinfectant solution, Aldasen 2000 [which removes bacteria including Tuberculous bacillus, fungus & viruses including Hep B, AIDS / HIV Viruses]
STAGE III: ULTRASONIC CLEANING
Ultrasonic cleaning is efficient in removal of dried whole blood, saliva, serum, bacterial plaque as well as dental cements from instruments surface. It works on the principle of cavitation, which has the ability to reach the tiniest crevice. This is especially important with difficult to clean items such as burs (dental drills used for removing caries) and serrated instruments.
STAGE IV: PACKING WITH DISPOSABLE POUCHES
The instruments are then segregated according to their usage & packed in disposable pouches. These pouches are color coded, which upon complete sterilization will change its color.
STAGE V: AUTOCLAVING PROCEDURE
All the package instruments are steam sterilized for 15min, at 121degrees celcius at 15 psi pressure. It acts by denaturation & coagulation of enzymes and proteins, which kills 99.99% of microorganisms rapidly.
When the instrument are completely sterilized the packages change color [Blue to Brown and Red to Yellow] Periodic monitoring of the autoclave is been carried out to check its efficiency. This is the currently recommended method by the American Dental Association.
STAGE VI: STORAGE
The autoclaved instruments [completely sterilized instruments] are stored in uv cabinet to prevent recontamination till further use.